Element sequencing of HiC library of Leptoglossus phyllopus : adult m... - SRA

SRX23987821: Element sequencing of HiC library of Leptoglossus phyllopus : adult male UT Arlington
1 ELEMENT (Element AVITI) run: 57.6M spots, 17.1G bases, 8.1Gb downloads

Design: Tissue was cross-linked, digested using the enzymes DdeI and DpnII, and proximity ligated. After proximity ligation and nucleic acid purification, DNA was sheared using the Diagenode Bioruptor Pico and size-selected to enrich for DNA fragments 200-600 bp. Following fragmentation and sample purification using SPRI-beads, library preparation was performed using the NEB Next Ultra II DNA Library Prep Kit and followed by the Element Biosciences Adept Library Prep Kit. The resulting HiC library was sequenced on one flow cell of an Element Biosciences Aviti system to 100x coverage of the genome size.

Submitted by: United States Department of Agriculture

Study: Genome sequencing of Leptoglossus phyllopus

PRJNA1089323 • SRP496266 • All experiments • All runs

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The purpose of this project is to sequence and assemble the genome of one female and one male Leptoglossus phyllopus, the Eastern leaf-footed bug, a widespread and conspicuous minor pest of many fruits, vegetables, grains, nuts and ornamentals.

Sample: Leptoglossus phyllopus male HiFi/HiC

SAMN40534740 • SRS20785296 • All experiments • All runs

Organism: Leptoglossus phyllopus

Library:

Name: ihLepPhyl2_male_Element_HiC

Instrument: Element AVITI

Strategy: Hi-C

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 57.6M spots, 17.1G bases, 8.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR28382978	57,574,692	17.1G	8.1Gb	2024-03-19

ID:: 32293751

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